Chromatin Immunoprecipitation:Article Title: A neurodegeneration checkpoint mediated by REST protects against the onset of Alzheimer’s disease
Article Snippet: .. The supernatant was diluted 1:5 in ChIP buffer (0.1% SDS, 1% Triton X-100, 2 mM EDTA, 140 mM NaCl, 50 mM Tris-HCl, pH 8) and precleared with 10 μl Protein A agarose beads (Life Technologies) that was equilibrated with 1% BSA. .. 2 μl of REST antibody, or non-specific IgG control antibody (Sigma, Catalog No. 17-641) was added to 20 μl of Protein A agarose beads in ChIP buffer and incubated for 2 h at room temperature to obtain REST-conjugated beads.
Article Title: A neurodegeneration checkpoint mediated by REST protects against the onset of Alzheimer's disease.
Article Snippet: .. The supernatant was diluted 1:5 in ChIP buffer (0.1% SDS, 1% Triton X100, 2mM EDTA, 140mMNaCl, 50mM Tris-HCl, pH 8) and precleared with 10 μl Protein A agarose beads (Life Technologies) that was equilibrated with 1% BSA. .. 2 μl of REST antibody, or non-specific IgG control antibody (Sigma, Catalog No. 17-641) was added to 20 μl of Protein A agarose beads in ChIP buffer and incubated for 2 h at room temperature to obtain REST-conjugated beads.
Article Title: Hominoid-specific transposable elements reshaped neural crest migration in craniofacial development
Article Snippet: .. Cells were resuspended in 1 mL ChIP buffer (150 mM NaCl, 1% Triton X-100, 500 mM DTT, 10 mM Tris-HCl, 5 mM EDTA) supplemented with Pierce protease inhibitor tablet and incubated on ice for 10 min. SDS was added to each sample to a final concentration of 0.3%, and chromatin was sheared using the following settings: 9 min, duty factor 5%, 6 °C in a Covaris M220 Focused-Ultrasonicator. ..
Article Title: Hominoid-specific transposable elements reshaped neural crest migration in craniofacial development.
Article Snippet: .. Cells were resuspended in 1 mL ChIP buffer (150 mM NaCl, 1% Triton X-100, 500 mM DTT, 10 mM Tris-HCl, 5 mM EDTA) supplemented with Pierce protease inhibitor tablet and incubated on ice for 10 min. SDS was added to each sample to a final concentration of 0.3%, and chromatin was sheared using the following settings: 9 min, duty factor 5%, 6 °C in a Covaris M220 Focused-Ultrasonicator. ..
Article Title: A novel long intergenic noncoding RNA indispensable for the formation of zygote in pig
Article Snippet: .. Antibody used: SUZ12(Abcam, ab12073); IgG (Millipore, CS200621) 2.14 Chromatin Immunoprecipitation (ChIP) assay We collected about 2,000 1-cell embryos and added 100 μl ChIP buffer (150 mM NaCl, 50 mM Tris–HCl pH 7.4, 1 mM EDTA, 0.1% SDS, 1% NP-40, 0.5% sodium deoxycholate, 0.5 mM DTT, 1 mM PMSF/cocktail) and 5 μl RNase inhibitor (Ambion, #AM2694) followed by incubation on ice for 10 min. We then harvested 10 μl of lysates used as inputs. .. The remaining lysates were divided into two parts and incubated with anti-H3K27me3 (Millipore, 07-499) or anti-H3K9me3 (Abcam, ab8898) antibody overnight at 4°C.
Article Title: YAP AND TEAD ARE TRANSCRIPTIONAL REGULATORS OF NEUROENDOCRINE DIFFERENTIATION AND GROWTH IN CARCINOID CELLS.
Article Snippet: First, protein-DNA interactions were reversibly crosslinked 178 by treating cells with 1% formaldehyde (Thermo Fisher Scientific) for 10 min, and 179 quenched with 125 mM glycine (BioShop Canada) for 5 min. .. Cells were washed four 180 times with cold PBS and lysed in ChIP buffer [50 mM Tris pH 7.5, 150 mM NaCl, 5 mM 181 EDTA, 1% NP-40, 1% Triton X-100, 0.1% SDS, 1X Halt Protease Inhibitor Cocktail 182 (Thermo Fisher Scientific)]. .. Chromatin was sheared with a Sonic Dismembrator Model 183 100 (Thermo Fisher Scientific) for 22-28 cycles (10s on/30s off).
Article Title: Human-specific transposable elements shaped the evolution of craniofacial development through regulation of neural crest migration
Article Snippet: .. Cells were resuspended in 1 mL ChIP buffer (150 mM NaCl, 1% Triton X-100, 500 mM DTT, 10 mM Tris-HCl, 5 mM EDTA) supplemented with Pierce protease inhibitor tablet and incubated on ice for 10 min. SDS was added to each sample to a final concentration of 0.3%, and chromatin was sheared using the following settings: 9 min, duty factor 5%, 6°C in a Covaris M220 Focused-Ultrasonicator. ..
Protease Inhibitor:Article Title: Hominoid-specific transposable elements reshaped neural crest migration in craniofacial development
Article Snippet: .. Cells were resuspended in 1 mL ChIP buffer (150 mM NaCl, 1% Triton X-100, 500 mM DTT, 10 mM Tris-HCl, 5 mM EDTA) supplemented with Pierce protease inhibitor tablet and incubated on ice for 10 min. SDS was added to each sample to a final concentration of 0.3%, and chromatin was sheared using the following settings: 9 min, duty factor 5%, 6 °C in a Covaris M220 Focused-Ultrasonicator. ..
Article Title: Hominoid-specific transposable elements reshaped neural crest migration in craniofacial development.
Article Snippet: .. Cells were resuspended in 1 mL ChIP buffer (150 mM NaCl, 1% Triton X-100, 500 mM DTT, 10 mM Tris-HCl, 5 mM EDTA) supplemented with Pierce protease inhibitor tablet and incubated on ice for 10 min. SDS was added to each sample to a final concentration of 0.3%, and chromatin was sheared using the following settings: 9 min, duty factor 5%, 6 °C in a Covaris M220 Focused-Ultrasonicator. ..
Article Title: YAP AND TEAD ARE TRANSCRIPTIONAL REGULATORS OF NEUROENDOCRINE DIFFERENTIATION AND GROWTH IN CARCINOID CELLS.
Article Snippet: First, protein-DNA interactions were reversibly crosslinked 178 by treating cells with 1% formaldehyde (Thermo Fisher Scientific) for 10 min, and 179 quenched with 125 mM glycine (BioShop Canada) for 5 min. .. Cells were washed four 180 times with cold PBS and lysed in ChIP buffer [50 mM Tris pH 7.5, 150 mM NaCl, 5 mM 181 EDTA, 1% NP-40, 1% Triton X-100, 0.1% SDS, 1X Halt Protease Inhibitor Cocktail 182 (Thermo Fisher Scientific)]. .. Chromatin was sheared with a Sonic Dismembrator Model 183 100 (Thermo Fisher Scientific) for 22-28 cycles (10s on/30s off).
Article Title: Human-specific transposable elements shaped the evolution of craniofacial development through regulation of neural crest migration
Article Snippet: .. Cells were resuspended in 1 mL ChIP buffer (150 mM NaCl, 1% Triton X-100, 500 mM DTT, 10 mM Tris-HCl, 5 mM EDTA) supplemented with Pierce protease inhibitor tablet and incubated on ice for 10 min. SDS was added to each sample to a final concentration of 0.3%, and chromatin was sheared using the following settings: 9 min, duty factor 5%, 6°C in a Covaris M220 Focused-Ultrasonicator. ..
Incubation:Article Title: Hominoid-specific transposable elements reshaped neural crest migration in craniofacial development
Article Snippet: .. Cells were resuspended in 1 mL ChIP buffer (150 mM NaCl, 1% Triton X-100, 500 mM DTT, 10 mM Tris-HCl, 5 mM EDTA) supplemented with Pierce protease inhibitor tablet and incubated on ice for 10 min. SDS was added to each sample to a final concentration of 0.3%, and chromatin was sheared using the following settings: 9 min, duty factor 5%, 6 °C in a Covaris M220 Focused-Ultrasonicator. ..
Article Title: Hominoid-specific transposable elements reshaped neural crest migration in craniofacial development.
Article Snippet: .. Cells were resuspended in 1 mL ChIP buffer (150 mM NaCl, 1% Triton X-100, 500 mM DTT, 10 mM Tris-HCl, 5 mM EDTA) supplemented with Pierce protease inhibitor tablet and incubated on ice for 10 min. SDS was added to each sample to a final concentration of 0.3%, and chromatin was sheared using the following settings: 9 min, duty factor 5%, 6 °C in a Covaris M220 Focused-Ultrasonicator. ..
Article Title: A novel long intergenic noncoding RNA indispensable for the formation of zygote in pig
Article Snippet: .. Antibody used: SUZ12(Abcam, ab12073); IgG (Millipore, CS200621) 2.14 Chromatin Immunoprecipitation (ChIP) assay We collected about 2,000 1-cell embryos and added 100 μl ChIP buffer (150 mM NaCl, 50 mM Tris–HCl pH 7.4, 1 mM EDTA, 0.1% SDS, 1% NP-40, 0.5% sodium deoxycholate, 0.5 mM DTT, 1 mM PMSF/cocktail) and 5 μl RNase inhibitor (Ambion, #AM2694) followed by incubation on ice for 10 min. We then harvested 10 μl of lysates used as inputs. .. The remaining lysates were divided into two parts and incubated with anti-H3K27me3 (Millipore, 07-499) or anti-H3K9me3 (Abcam, ab8898) antibody overnight at 4°C.
Article Title: Human-specific transposable elements shaped the evolution of craniofacial development through regulation of neural crest migration
Article Snippet: .. Cells were resuspended in 1 mL ChIP buffer (150 mM NaCl, 1% Triton X-100, 500 mM DTT, 10 mM Tris-HCl, 5 mM EDTA) supplemented with Pierce protease inhibitor tablet and incubated on ice for 10 min. SDS was added to each sample to a final concentration of 0.3%, and chromatin was sheared using the following settings: 9 min, duty factor 5%, 6°C in a Covaris M220 Focused-Ultrasonicator. ..
Concentration Assay:Article Title: Hominoid-specific transposable elements reshaped neural crest migration in craniofacial development
Article Snippet: .. Cells were resuspended in 1 mL ChIP buffer (150 mM NaCl, 1% Triton X-100, 500 mM DTT, 10 mM Tris-HCl, 5 mM EDTA) supplemented with Pierce protease inhibitor tablet and incubated on ice for 10 min. SDS was added to each sample to a final concentration of 0.3%, and chromatin was sheared using the following settings: 9 min, duty factor 5%, 6 °C in a Covaris M220 Focused-Ultrasonicator. ..
Article Title: Hominoid-specific transposable elements reshaped neural crest migration in craniofacial development.
Article Snippet: .. Cells were resuspended in 1 mL ChIP buffer (150 mM NaCl, 1% Triton X-100, 500 mM DTT, 10 mM Tris-HCl, 5 mM EDTA) supplemented with Pierce protease inhibitor tablet and incubated on ice for 10 min. SDS was added to each sample to a final concentration of 0.3%, and chromatin was sheared using the following settings: 9 min, duty factor 5%, 6 °C in a Covaris M220 Focused-Ultrasonicator. ..
Article Title: Human-specific transposable elements shaped the evolution of craniofacial development through regulation of neural crest migration
Article Snippet: .. Cells were resuspended in 1 mL ChIP buffer (150 mM NaCl, 1% Triton X-100, 500 mM DTT, 10 mM Tris-HCl, 5 mM EDTA) supplemented with Pierce protease inhibitor tablet and incubated on ice for 10 min. SDS was added to each sample to a final concentration of 0.3%, and chromatin was sheared using the following settings: 9 min, duty factor 5%, 6°C in a Covaris M220 Focused-Ultrasonicator. ..
other:
|
Thermo Fisher is a verified supplier 
Thermo Fisher manufactures this product 